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  • ✇Ars Technica - All content
  • How the perils of space have affected asteroid RyuguElizabeth Rayne
    Enlarge / The surface of Ryugu. Image credit: JAXA, University of Tokyo, Kochi University, Rikkyo University, Nagoya University, Chiba Institute of Technology, Meiji University, Aizu University, AIST (credit: JAXA) An asteroid that has been wandering through space for billions of years is going to have been bombarded by everything from rocks to radiation. Billions of years traveling through interplanetary space increase the odds of colliding with something in the vast empti
     

How the perils of space have affected asteroid Ryugu

19. Květen 2024 v 13:55
Grey image of a complicated surface composed of many small rocks bound together by dust.

Enlarge / The surface of Ryugu. Image credit: JAXA, University of Tokyo, Kochi University, Rikkyo University, Nagoya University, Chiba Institute of Technology, Meiji University, Aizu University, AIST (credit: JAXA)

An asteroid that has been wandering through space for billions of years is going to have been bombarded by everything from rocks to radiation. Billions of years traveling through interplanetary space increase the odds of colliding with something in the vast emptiness, and at least one of those impacts had enough force to leave the asteroid Ryugu forever changed.

When the Japanese Space Agency’s Hayabusa2 spacecraft touched down on Ryugu, it collected samples from the surface that revealed that particles of magnetite (which is usually magnetic) in the asteroid’s regolith are devoid of magnetism. A team of researchers from Hokkaido University and several other institutions in Japan are now offering an explanation for how this material lost most of its magnetic properties. Their analysis showed that it was caused by at least one high-velocity micrometeoroid collision that broke the magnetite’s chemical structure down so that it was no longer magnetic.

“We surmised that pseudo-magnetite was created [as] the result of space weathering by micrometeoroid impact,” the researchers, led by Hokkaido University professor Yuki Kimura, said in a study recently published in Nature Communications.

Read 12 remaining paragraphs | Comments

  • ✇Ars Technica - All content
  • How the Moon got a makeoverElizabeth Rayne
    Enlarge (credit: NASA Goddard/ASU) Our Moon may appear to shine peacefully in the night sky, but billions of years ago, it was given a facial by volcanic turmoil. One question that has gone unanswered for decades is why there are more titanium-rich volcanic rocks, such as ilmenite, on the near side as opposed to the far side. Now a team of researchers at Arizona Lunar and Planetary Laboratory are proposing a possible explanation for that. The lunar surface was once flooded by
     

How the Moon got a makeover

11. Květen 2024 v 12:00
Image of the moon.

Enlarge (credit: NASA Goddard/ASU)

Our Moon may appear to shine peacefully in the night sky, but billions of years ago, it was given a facial by volcanic turmoil.

One question that has gone unanswered for decades is why there are more titanium-rich volcanic rocks, such as ilmenite, on the near side as opposed to the far side. Now a team of researchers at Arizona Lunar and Planetary Laboratory are proposing a possible explanation for that.

The lunar surface was once flooded by a bubbling magma ocean, and after the magma ocean had hardened, there was an enormous impact on the far side. Heat from this impact spread to the near side and made the crust unstable, causing sheets of heavier and denser minerals on the surface to gradually sink deep into the mantle. These melted again and were belched out by volcanoes. Lava from these eruptions (more of which happened on the near side) ended up in what are now titanium-rich flows of volcanic rock. In other words, the Moon’s old face vanished, only to resurface.

Read 12 remaining paragraphs | Comments

  • ✇Ars Technica - All content
  • Io: New image of a lake of fire, signs of permanent volcanismJohn Timmer
    Enlarge (credit: NASA/JPL-Caltech/SwRI/MSSS/Gerald Eichstädt/Thomas Thomopoulos) Ever since the Voyager mission sent home images of Jupiter's moon Io spewing material into space, we've gradually built up a clearer picture of Io's volcanic activity. It slowly became clear that Io, which is a bit smaller than Mercury, is the most volcanically active body in the Solar System, with all that activity driven by the gravitational strain caused by Jupiter and its three other giant mo
     

Io: New image of a lake of fire, signs of permanent volcanism

19. Duben 2024 v 20:17
Io: New image of a lake of fire, signs of permanent volcanism

Enlarge (credit: NASA/JPL-Caltech/SwRI/MSSS/Gerald Eichstädt/Thomas Thomopoulos)

Ever since the Voyager mission sent home images of Jupiter's moon Io spewing material into space, we've gradually built up a clearer picture of Io's volcanic activity. It slowly became clear that Io, which is a bit smaller than Mercury, is the most volcanically active body in the Solar System, with all that activity driven by the gravitational strain caused by Jupiter and its three other giant moons. There is so much volcanism that its surface has been completely remodeled, with no signs of impact craters.

A few more details about its violence came to light this week, with new images being released of the moon's features, including an island in a lake of lava, taken by the Juno orbiter. At the same time, imaging done using an Earth-based telescope has provided some indications that this volcanism has been reshaping Io from almost the moment it formed.

Fiery, glassy lakes

The Juno orbiter's mission is primarily focused on studying Jupiter, including the dynamics of its storms and its internal composition. But many of its orbital passes have taken it right past Io, and this week, the Jet Propulsion Laboratory released some of the best images from these flybys. They include a shot of Loki Patera, a lake of lava that has an island within it. Also featured: the impossibly sheer slopes of Io's Steeple Mountain.

Read 12 remaining paragraphs | Comments

  • ✇MIT News - Nanoscience and nanotechnology | MIT.nano
  • How to prevent biofilms in spaceDavid L. Chandler | MIT News
    After exposure in space aboard the International Space Station, a new kind of surface treatment significantly reduced the growth of biofilms, scientists report. Biofilms are mats of microbial or fungal growth that can clog hoses or filters in water processing systems, or potentially cause illness in people. In the experiment, researchers investigated a variety of surfaces treated in different ways and exposed to a bacteria called Pseudomonas aeruginosa, which is an opportunistic pathogen than
     

How to prevent biofilms in space

After exposure in space aboard the International Space Station, a new kind of surface treatment significantly reduced the growth of biofilms, scientists report. Biofilms are mats of microbial or fungal growth that can clog hoses or filters in water processing systems, or potentially cause illness in people.

In the experiment, researchers investigated a variety of surfaces treated in different ways and exposed to a bacteria called Pseudomonas aeruginosa, which is an opportunistic pathogen than can cause infections in humans, especially in hospitals. The surfaces were incubated for three days aboard the space station, starting in 2019. The results show that textured surfaces impregnated with a lubricant were highly successful at preventing biofilm growth during their long exposure in space. The findings are described in a paper in the journal Nature Microgravity, by Samantha McBride PhD ’20 and Kripa Varanasi of MIT, Pamela Flores and Luis Zea at the University of Colorado, and Jonathan Galakza at NASA Ames Research Center.

Clogs in water recovery system hoses aboard the ISS have been so severe at times, the hoses had to be sent back to Earth for cleaning and refurbishing. And while it isn’t known whether biofilms have directly contributed to astronaut illnesses, on Earth, biofilms are associated with 65 percent of microbial infections, and 80 percent of chronic infections, the researchers say.

One approach to preventing biofilms is to use surfaces coated with certain metals or oxides that kill microbes, but this approach can fail when a layer of dead microbes builds up on the surface and allows biofilm to form above it. But this was not the case with the liquid-infused surface that performed well in the ISS experiments: Rather than killing the microbes, it prevented them from adhering to the surface in the first place.

The specific surface used was made of silicon that was etched to produce a nanoscale forest of pillars. This spiky surface is then infused with a silicon oil, which is drawn into the texture and held in place by capillary action, leaving a smooth and highly slippery surface that significantly reduces the adhesion of microbes and prevents them from forming a biofilm.

Identical experiments were conducted on Earth as well as on the space station to determine the differences produced by the microgravity environment in orbit. To the researchers' surprise, the liquid-infused surface performed even better in space than it did on Earth at preventing microbial adhesion.

On previous and current space stations, including the USSR’s Mir station, Salyut 6, and Salyut 7,  as well as the International Space Station, “they’ve seen these biofilms, and they jeopardize a variety of instruments or equipment, including space suits, recycling units, radiators, and water treatment facilities, so it’s a very important problem that needed to be understood,” says Varanasi, a professor of mechanical engineering and founder of a company called LiquiGlide, which makes liquid-impregnated surfaces for containers to help their contents slide out.

Previous tests on Earth had shown that these treated surfaces could significantly reduce biofilm adhesion. When the samples from the space station were retrieved and tested, “we found that these surfaces are extremely good at preventing biofilm formation in the space station as well,” Varanasi says. This is important because past work has found that microgravity can have a significant influence on biofilm morphologies, attachment behavior, and gene expression, according to McBride. Thus, strategies that work well on Earth for biofilm mitigation may not necessarily be applicable to microgravity situations.

Preventing biofilms will be especially important for future long-duration missions, such as to the moon or Mars, where the option of quickly returning fouled equipment or sick astronauts to Earth will not be available, the team says. If further testing confirms its long-term stability and successful biofilm prevention, coatings based on the liquid-treated surface concept could be applied to a variety of critical components that are known to be susceptible to biofilm fouling, such as water treatment hoses and filters, or to parts that come in close contact with astronauts, such as gloves or food preparation surfaces.

In the terrestrial samples, biofilm formation was reduced by about 74 percent, while the space station samples showed a reduction of about 86 percent, says Flores, who did much of the testing of the ISS-exposed samples. “The results we got were surprising,” she says, because earlier tests carried out by others had shown biofilm formation was actually greater in space than on Earth. “We actually found the opposite on these samples,” she says.

While the tests used a specific and well-studied gram-negative kind of bacteria, she says, the results should apply to any kind of gram-negative bacteria, and likely to gram-positive bacteria as well. They found that the areas of the surface where no bacterial growth took place were covered by a thin layer of nucleic acids, which have a slight negative electric charge that may have helped to prevent microbes from adhering. Both gram-positive and gram-negative bacteria have a slight negative charge, which could repel them from that negatively charged surface, Flores says.

Other types of anti-fouling surfaces, Varanasi says, “work mostly on a biocidal property, which usually only works for a first layer of cells because after those cells die they can form a deposit, and microbes can grow on top of them. So, usually it’s been a very hard problem.” But with the liquid-impregnated surface, where what is exposed is mostly just the liquid itself, there are very few defects or points where the bacteria can find a footing, he says.

Although the test material was on the space station for more than a year, the actual tests were only performed over a three-day period because they required active participation by the astronauts whose schedules are always very busy. But one recommendation the team has made, based on these initial results, is that longer-duration tests should be carried out on a future mission. In these first tests, Flores says, the results after the third day looked the same as after the first and second days. “We don’t know for how long it will be able to keep up this performance, so we definitely recommend a longer time of incubation, and also, if possible, a continuous analysis, and not just end points.”

Zea, who initiated the project with NASA, says that this was the first time the agency has conducted tests that involved joint participation by two of its science programs, biology and physical sciences. “I think it stresses the importance of multidisciplinarity because we need to be able to combine these different disciplines to find solutions to real world problems.”

Biofilms are also a significant medical issue on Earth, especially on medical devices or implants including catheters, where they can lead to significant disease problems. The same kind of liquid-impregnated surfaces may have a role to play in helping to address these issues, Varanasi says.

The project was supported by NASA and used facilities provided by several other companies and organizations.

© Image: Space Biofilm Program

Inside these vials are chambers containing the new surface material and the microbes. They were launched in stasis to ISS to avoid bacterial growth before reaching microgravity conditions. Once in ISS, the astronauts activated the samples by combining the various chambers in the vials.
  • ✇MIT News - Nanoscience and nanotechnology | MIT.nano
  • How to prevent biofilms in spaceDavid L. Chandler | MIT News
    After exposure in space aboard the International Space Station, a new kind of surface treatment significantly reduced the growth of biofilms, scientists report. Biofilms are mats of microbial or fungal growth that can clog hoses or filters in water processing systems, or potentially cause illness in people. In the experiment, researchers investigated a variety of surfaces treated in different ways and exposed to a bacteria called Pseudomonas aeruginosa, which is an opportunistic pathogen than
     

How to prevent biofilms in space

After exposure in space aboard the International Space Station, a new kind of surface treatment significantly reduced the growth of biofilms, scientists report. Biofilms are mats of microbial or fungal growth that can clog hoses or filters in water processing systems, or potentially cause illness in people.

In the experiment, researchers investigated a variety of surfaces treated in different ways and exposed to a bacteria called Pseudomonas aeruginosa, which is an opportunistic pathogen than can cause infections in humans, especially in hospitals. The surfaces were incubated for three days aboard the space station, starting in 2019. The results show that textured surfaces impregnated with a lubricant were highly successful at preventing biofilm growth during their long exposure in space. The findings are described in a paper in the journal Nature Microgravity, by Samantha McBride PhD ’20 and Kripa Varanasi of MIT, Pamela Flores and Luis Zea at the University of Colorado, and Jonathan Galakza at NASA Ames Research Center.

Clogs in water recovery system hoses aboard the ISS have been so severe at times, the hoses had to be sent back to Earth for cleaning and refurbishing. And while it isn’t known whether biofilms have directly contributed to astronaut illnesses, on Earth, biofilms are associated with 65 percent of microbial infections, and 80 percent of chronic infections, the researchers say.

One approach to preventing biofilms is to use surfaces coated with certain metals or oxides that kill microbes, but this approach can fail when a layer of dead microbes builds up on the surface and allows biofilm to form above it. But this was not the case with the liquid-infused surface that performed well in the ISS experiments: Rather than killing the microbes, it prevented them from adhering to the surface in the first place.

The specific surface used was made of silicon that was etched to produce a nanoscale forest of pillars. This spiky surface is then infused with a silicon oil, which is drawn into the texture and held in place by capillary action, leaving a smooth and highly slippery surface that significantly reduces the adhesion of microbes and prevents them from forming a biofilm.

Identical experiments were conducted on Earth as well as on the space station to determine the differences produced by the microgravity environment in orbit. To the researchers' surprise, the liquid-infused surface performed even better in space than it did on Earth at preventing microbial adhesion.

On previous and current space stations, including the USSR’s Mir station, Salyut 6, and Salyut 7,  as well as the International Space Station, “they’ve seen these biofilms, and they jeopardize a variety of instruments or equipment, including space suits, recycling units, radiators, and water treatment facilities, so it’s a very important problem that needed to be understood,” says Varanasi, a professor of mechanical engineering and founder of a company called LiquiGlide, which makes liquid-impregnated surfaces for containers to help their contents slide out.

Previous tests on Earth had shown that these treated surfaces could significantly reduce biofilm adhesion. When the samples from the space station were retrieved and tested, “we found that these surfaces are extremely good at preventing biofilm formation in the space station as well,” Varanasi says. This is important because past work has found that microgravity can have a significant influence on biofilm morphologies, attachment behavior, and gene expression, according to McBride. Thus, strategies that work well on Earth for biofilm mitigation may not necessarily be applicable to microgravity situations.

Preventing biofilms will be especially important for future long-duration missions, such as to the moon or Mars, where the option of quickly returning fouled equipment or sick astronauts to Earth will not be available, the team says. If further testing confirms its long-term stability and successful biofilm prevention, coatings based on the liquid-treated surface concept could be applied to a variety of critical components that are known to be susceptible to biofilm fouling, such as water treatment hoses and filters, or to parts that come in close contact with astronauts, such as gloves or food preparation surfaces.

In the terrestrial samples, biofilm formation was reduced by about 74 percent, while the space station samples showed a reduction of about 86 percent, says Flores, who did much of the testing of the ISS-exposed samples. “The results we got were surprising,” she says, because earlier tests carried out by others had shown biofilm formation was actually greater in space than on Earth. “We actually found the opposite on these samples,” she says.

While the tests used a specific and well-studied gram-negative kind of bacteria, she says, the results should apply to any kind of gram-negative bacteria, and likely to gram-positive bacteria as well. They found that the areas of the surface where no bacterial growth took place were covered by a thin layer of nucleic acids, which have a slight negative electric charge that may have helped to prevent microbes from adhering. Both gram-positive and gram-negative bacteria have a slight negative charge, which could repel them from that negatively charged surface, Flores says.

Other types of anti-fouling surfaces, Varanasi says, “work mostly on a biocidal property, which usually only works for a first layer of cells because after those cells die they can form a deposit, and microbes can grow on top of them. So, usually it’s been a very hard problem.” But with the liquid-impregnated surface, where what is exposed is mostly just the liquid itself, there are very few defects or points where the bacteria can find a footing, he says.

Although the test material was on the space station for more than a year, the actual tests were only performed over a three-day period because they required active participation by the astronauts whose schedules are always very busy. But one recommendation the team has made, based on these initial results, is that longer-duration tests should be carried out on a future mission. In these first tests, Flores says, the results after the third day looked the same as after the first and second days. “We don’t know for how long it will be able to keep up this performance, so we definitely recommend a longer time of incubation, and also, if possible, a continuous analysis, and not just end points.”

Zea, who initiated the project with NASA, says that this was the first time the agency has conducted tests that involved joint participation by two of its science programs, biology and physical sciences. “I think it stresses the importance of multidisciplinarity because we need to be able to combine these different disciplines to find solutions to real world problems.”

Biofilms are also a significant medical issue on Earth, especially on medical devices or implants including catheters, where they can lead to significant disease problems. The same kind of liquid-impregnated surfaces may have a role to play in helping to address these issues, Varanasi says.

The project was supported by NASA and used facilities provided by several other companies and organizations.

© Image: Space Biofilm Program

Inside these vials are chambers containing the new surface material and the microbes. They were launched in stasis to ISS to avoid bacterial growth before reaching microgravity conditions. Once in ISS, the astronauts activated the samples by combining the various chambers in the vials.
  • ✇MIT News - Nanoscience and nanotechnology | MIT.nano
  • How to prevent biofilms in spaceDavid L. Chandler | MIT News
    After exposure in space aboard the International Space Station, a new kind of surface treatment significantly reduced the growth of biofilms, scientists report. Biofilms are mats of microbial or fungal growth that can clog hoses or filters in water processing systems, or potentially cause illness in people. In the experiment, researchers investigated a variety of surfaces treated in different ways and exposed to a bacteria called Pseudomonas aeruginosa, which is an opportunistic pathogen than
     

How to prevent biofilms in space

After exposure in space aboard the International Space Station, a new kind of surface treatment significantly reduced the growth of biofilms, scientists report. Biofilms are mats of microbial or fungal growth that can clog hoses or filters in water processing systems, or potentially cause illness in people.

In the experiment, researchers investigated a variety of surfaces treated in different ways and exposed to a bacteria called Pseudomonas aeruginosa, which is an opportunistic pathogen than can cause infections in humans, especially in hospitals. The surfaces were incubated for three days aboard the space station, starting in 2019. The results show that textured surfaces impregnated with a lubricant were highly successful at preventing biofilm growth during their long exposure in space. The findings are described in a paper in the journal Nature Microgravity, by Samantha McBride PhD ’20 and Kripa Varanasi of MIT, Pamela Flores and Luis Zea at the University of Colorado, and Jonathan Galakza at NASA Ames Research Center.

Clogs in water recovery system hoses aboard the ISS have been so severe at times, the hoses had to be sent back to Earth for cleaning and refurbishing. And while it isn’t known whether biofilms have directly contributed to astronaut illnesses, on Earth, biofilms are associated with 65 percent of microbial infections, and 80 percent of chronic infections, the researchers say.

One approach to preventing biofilms is to use surfaces coated with certain metals or oxides that kill microbes, but this approach can fail when a layer of dead microbes builds up on the surface and allows biofilm to form above it. But this was not the case with the liquid-infused surface that performed well in the ISS experiments: Rather than killing the microbes, it prevented them from adhering to the surface in the first place.

The specific surface used was made of silicon that was etched to produce a nanoscale forest of pillars. This spiky surface is then infused with a silicon oil, which is drawn into the texture and held in place by capillary action, leaving a smooth and highly slippery surface that significantly reduces the adhesion of microbes and prevents them from forming a biofilm.

Identical experiments were conducted on Earth as well as on the space station to determine the differences produced by the microgravity environment in orbit. To the researchers' surprise, the liquid-infused surface performed even better in space than it did on Earth at preventing microbial adhesion.

On previous and current space stations, including the USSR’s Mir station, Salyut 6, and Salyut 7,  as well as the International Space Station, “they’ve seen these biofilms, and they jeopardize a variety of instruments or equipment, including space suits, recycling units, radiators, and water treatment facilities, so it’s a very important problem that needed to be understood,” says Varanasi, a professor of mechanical engineering and founder of a company called LiquiGlide, which makes liquid-impregnated surfaces for containers to help their contents slide out.

Previous tests on Earth had shown that these treated surfaces could significantly reduce biofilm adhesion. When the samples from the space station were retrieved and tested, “we found that these surfaces are extremely good at preventing biofilm formation in the space station as well,” Varanasi says. This is important because past work has found that microgravity can have a significant influence on biofilm morphologies, attachment behavior, and gene expression, according to McBride. Thus, strategies that work well on Earth for biofilm mitigation may not necessarily be applicable to microgravity situations.

Preventing biofilms will be especially important for future long-duration missions, such as to the moon or Mars, where the option of quickly returning fouled equipment or sick astronauts to Earth will not be available, the team says. If further testing confirms its long-term stability and successful biofilm prevention, coatings based on the liquid-treated surface concept could be applied to a variety of critical components that are known to be susceptible to biofilm fouling, such as water treatment hoses and filters, or to parts that come in close contact with astronauts, such as gloves or food preparation surfaces.

In the terrestrial samples, biofilm formation was reduced by about 74 percent, while the space station samples showed a reduction of about 86 percent, says Flores, who did much of the testing of the ISS-exposed samples. “The results we got were surprising,” she says, because earlier tests carried out by others had shown biofilm formation was actually greater in space than on Earth. “We actually found the opposite on these samples,” she says.

While the tests used a specific and well-studied gram-negative kind of bacteria, she says, the results should apply to any kind of gram-negative bacteria, and likely to gram-positive bacteria as well. They found that the areas of the surface where no bacterial growth took place were covered by a thin layer of nucleic acids, which have a slight negative electric charge that may have helped to prevent microbes from adhering. Both gram-positive and gram-negative bacteria have a slight negative charge, which could repel them from that negatively charged surface, Flores says.

Other types of anti-fouling surfaces, Varanasi says, “work mostly on a biocidal property, which usually only works for a first layer of cells because after those cells die they can form a deposit, and microbes can grow on top of them. So, usually it’s been a very hard problem.” But with the liquid-impregnated surface, where what is exposed is mostly just the liquid itself, there are very few defects or points where the bacteria can find a footing, he says.

Although the test material was on the space station for more than a year, the actual tests were only performed over a three-day period because they required active participation by the astronauts whose schedules are always very busy. But one recommendation the team has made, based on these initial results, is that longer-duration tests should be carried out on a future mission. In these first tests, Flores says, the results after the third day looked the same as after the first and second days. “We don’t know for how long it will be able to keep up this performance, so we definitely recommend a longer time of incubation, and also, if possible, a continuous analysis, and not just end points.”

Zea, who initiated the project with NASA, says that this was the first time the agency has conducted tests that involved joint participation by two of its science programs, biology and physical sciences. “I think it stresses the importance of multidisciplinarity because we need to be able to combine these different disciplines to find solutions to real world problems.”

Biofilms are also a significant medical issue on Earth, especially on medical devices or implants including catheters, where they can lead to significant disease problems. The same kind of liquid-impregnated surfaces may have a role to play in helping to address these issues, Varanasi says.

The project was supported by NASA and used facilities provided by several other companies and organizations.

© Image: Space Biofilm Program

Inside these vials are chambers containing the new surface material and the microbes. They were launched in stasis to ISS to avoid bacterial growth before reaching microgravity conditions. Once in ISS, the astronauts activated the samples by combining the various chambers in the vials.
  • ✇MIT News - Nanoscience and nanotechnology | MIT.nano
  • How to prevent biofilms in spaceDavid L. Chandler | MIT News
    After exposure in space aboard the International Space Station, a new kind of surface treatment significantly reduced the growth of biofilms, scientists report. Biofilms are mats of microbial or fungal growth that can clog hoses or filters in water processing systems, or potentially cause illness in people. In the experiment, researchers investigated a variety of surfaces treated in different ways and exposed to a bacteria called Pseudomonas aeruginosa, which is an opportunistic pathogen than
     

How to prevent biofilms in space

After exposure in space aboard the International Space Station, a new kind of surface treatment significantly reduced the growth of biofilms, scientists report. Biofilms are mats of microbial or fungal growth that can clog hoses or filters in water processing systems, or potentially cause illness in people.

In the experiment, researchers investigated a variety of surfaces treated in different ways and exposed to a bacteria called Pseudomonas aeruginosa, which is an opportunistic pathogen than can cause infections in humans, especially in hospitals. The surfaces were incubated for three days aboard the space station, starting in 2019. The results show that textured surfaces impregnated with a lubricant were highly successful at preventing biofilm growth during their long exposure in space. The findings are described in a paper in the journal Nature Microgravity, by Samantha McBride PhD ’20 and Kripa Varanasi of MIT, Pamela Flores and Luis Zea at the University of Colorado, and Jonathan Galakza at NASA Ames Research Center.

Clogs in water recovery system hoses aboard the ISS have been so severe at times, the hoses had to be sent back to Earth for cleaning and refurbishing. And while it isn’t known whether biofilms have directly contributed to astronaut illnesses, on Earth, biofilms are associated with 65 percent of microbial infections, and 80 percent of chronic infections, the researchers say.

One approach to preventing biofilms is to use surfaces coated with certain metals or oxides that kill microbes, but this approach can fail when a layer of dead microbes builds up on the surface and allows biofilm to form above it. But this was not the case with the liquid-infused surface that performed well in the ISS experiments: Rather than killing the microbes, it prevented them from adhering to the surface in the first place.

The specific surface used was made of silicon that was etched to produce a nanoscale forest of pillars. This spiky surface is then infused with a silicon oil, which is drawn into the texture and held in place by capillary action, leaving a smooth and highly slippery surface that significantly reduces the adhesion of microbes and prevents them from forming a biofilm.

Identical experiments were conducted on Earth as well as on the space station to determine the differences produced by the microgravity environment in orbit. To the researchers' surprise, the liquid-infused surface performed even better in space than it did on Earth at preventing microbial adhesion.

On previous and current space stations, including the USSR’s Mir station, Salyut 6, and Salyut 7,  as well as the International Space Station, “they’ve seen these biofilms, and they jeopardize a variety of instruments or equipment, including space suits, recycling units, radiators, and water treatment facilities, so it’s a very important problem that needed to be understood,” says Varanasi, a professor of mechanical engineering and founder of a company called LiquiGlide, which makes liquid-impregnated surfaces for containers to help their contents slide out.

Previous tests on Earth had shown that these treated surfaces could significantly reduce biofilm adhesion. When the samples from the space station were retrieved and tested, “we found that these surfaces are extremely good at preventing biofilm formation in the space station as well,” Varanasi says. This is important because past work has found that microgravity can have a significant influence on biofilm morphologies, attachment behavior, and gene expression, according to McBride. Thus, strategies that work well on Earth for biofilm mitigation may not necessarily be applicable to microgravity situations.

Preventing biofilms will be especially important for future long-duration missions, such as to the moon or Mars, where the option of quickly returning fouled equipment or sick astronauts to Earth will not be available, the team says. If further testing confirms its long-term stability and successful biofilm prevention, coatings based on the liquid-treated surface concept could be applied to a variety of critical components that are known to be susceptible to biofilm fouling, such as water treatment hoses and filters, or to parts that come in close contact with astronauts, such as gloves or food preparation surfaces.

In the terrestrial samples, biofilm formation was reduced by about 74 percent, while the space station samples showed a reduction of about 86 percent, says Flores, who did much of the testing of the ISS-exposed samples. “The results we got were surprising,” she says, because earlier tests carried out by others had shown biofilm formation was actually greater in space than on Earth. “We actually found the opposite on these samples,” she says.

While the tests used a specific and well-studied gram-negative kind of bacteria, she says, the results should apply to any kind of gram-negative bacteria, and likely to gram-positive bacteria as well. They found that the areas of the surface where no bacterial growth took place were covered by a thin layer of nucleic acids, which have a slight negative electric charge that may have helped to prevent microbes from adhering. Both gram-positive and gram-negative bacteria have a slight negative charge, which could repel them from that negatively charged surface, Flores says.

Other types of anti-fouling surfaces, Varanasi says, “work mostly on a biocidal property, which usually only works for a first layer of cells because after those cells die they can form a deposit, and microbes can grow on top of them. So, usually it’s been a very hard problem.” But with the liquid-impregnated surface, where what is exposed is mostly just the liquid itself, there are very few defects or points where the bacteria can find a footing, he says.

Although the test material was on the space station for more than a year, the actual tests were only performed over a three-day period because they required active participation by the astronauts whose schedules are always very busy. But one recommendation the team has made, based on these initial results, is that longer-duration tests should be carried out on a future mission. In these first tests, Flores says, the results after the third day looked the same as after the first and second days. “We don’t know for how long it will be able to keep up this performance, so we definitely recommend a longer time of incubation, and also, if possible, a continuous analysis, and not just end points.”

Zea, who initiated the project with NASA, says that this was the first time the agency has conducted tests that involved joint participation by two of its science programs, biology and physical sciences. “I think it stresses the importance of multidisciplinarity because we need to be able to combine these different disciplines to find solutions to real world problems.”

Biofilms are also a significant medical issue on Earth, especially on medical devices or implants including catheters, where they can lead to significant disease problems. The same kind of liquid-impregnated surfaces may have a role to play in helping to address these issues, Varanasi says.

The project was supported by NASA and used facilities provided by several other companies and organizations.

© Image: Space Biofilm Program

Inside these vials are chambers containing the new surface material and the microbes. They were launched in stasis to ISS to avoid bacterial growth before reaching microgravity conditions. Once in ISS, the astronauts activated the samples by combining the various chambers in the vials.
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